Name: China High Quality Tiron Powder Manufacturers Suppliers - Factory Direct Wholesale - HUILIN BIO
Brand: Huilin
SKU: 287180051

Introduction: What is Tiron?

Tiron, systematically known as disodium 4,5-dihydroxy-1,3-benzenedisulfonate, is a versatile and water-soluble organic compound. Its unique molecular structure features a catechol (ortho-dihydroxybenzene) core with two sulfonate groups, making it an exceptional chelating agent, particularly for transition metal ions like iron (Fe³⁺) and titanium (Ti⁴⁺).

For decades, Tiron has been a cornerstone reagent in analytical chemistry, renowned for forming stable, water-soluble colored complexes with specific metals, enabling their sensitive detection and quantification. Beyond its traditional role, modern research has unlocked its significant potential as a potent, cell-permeable antioxidant in biological systems, effectively scavenging harmful free radicals like hydroxyl radicals and superoxide anions.

Tiron

Biochemical & Physical Parameters

This table summarizes the essential data for handling and formulating with Tiron Powder.

Parameter	Value / Description
Physical Form	White to light gray-yellow powder
Melting Point	> 300 °C
Solubility	Highly soluble in water. Up to 1350 g/L reported. Slightly soluble in ethanol. Insoluble in acetone.
pH (10 g/L)	~5.0
Storage Conditions	Store in a sealed container in a cool, dry place at room temperature.
Stability	Stable under recommended conditions. Solutions may discolor upon prolonged standing, indicating potential degradation.

Mechanism of Action: How Tiron Works

The efficacy of Tiron stems from its dual-functional molecular design:

1. Potent Metal Chelation:

The catechol group in Tiron provides two oxygen atoms in an ortho configuration, which forms exceptionally strong, octahedral coordination complexes with metal cations. This chelation is highly selective, especially for Fe³⁺ and Ti⁴⁺, forming deep blue and orange complexes, respectively. This action effectively sequesters ("masks") free metal ions, preventing them from participating in unwanted reactions like Fenton chemistry, which generates damaging free radicals.

2. Direct Free Radical Scavenging:

Independently of metal chelation, the Tiron molecule acts as a direct electron donor. It neutralizes reactive oxygen species (ROS) such as superoxide anions (O₂•⁻) and hydroxyl radicals (•OH), converting them into less harmful molecules. This makes it a valuable tool for studying oxidative stress pathways in biological research.

Key Benefits of Choosing Our Tiron Powder

High Purity & Consistency: We guarantee a minimum purity of 99%, ensuring reliable and reproducible results in your sensitive analytical or research applications.
Exceptional Water Solubility: The sulfonate groups grant Tiron outstanding solubility in aqueous buffers, simplifying experimental setup and formulation without the need for organic co-solvents.
Dual-Action Functionality: Serves as both a specific metal chelator and a broad-spectrum antioxidant, offering versatility across chemistry and life science projects.
Proven Research Utility: A well-cited, classic reagent with established protocols for metal analysis and a growing reputation in oxidative stress research, providing a trusted foundation for your work.

Primary Applications

Tiron Chemical is a multi-disciplinary tool with critical roles in:

1. Analytical Chemistry:

Spectrophotometric Determination: A classic chromogenic agent for the precise colorimetric detection and quantification of Titanium (Ti), Iron (Fe), and Molybdenum (Mo).
Complexometric Titration: Used as a metal indicator in the titration of iron.
Metal Masking Agent: Selectively binds to interfering metal ions in solution, allowing for the analysis of other components without interference.

2. Biochemical & Life Science Research:

Oxidative Stress Studies: Widely used as a scavenger of superoxide and hydroxyl radicals to investigate their role in cell signaling, apoptosis, and disease models.
Metal Biology: Used to chelate labile iron pools in cells, helping to elucidate the role of free iron in metabolic processes and toxicity.
Cytoprotection: Studied for its potential to protect cells from oxidative damage induced by toxins or environmental stress.

3. Industrial Uses:

Synthetic Intermediate: Serves as a building block in organic synthesis and for the preparation of pharmaceutical active ingredients (APIs).
Formulation Additive: Potential use as a stabilizing antioxidant or metal-sequestering agent in various formulations.

Applications of Tiron

FAQ: Frequently Asked Questions

Q: What is the difference between the reported molecular weights (e.g., 294.2, 314.2, 332.2)?

A: The variation stems from the form of the compound. The basic disodium salt (C₆H₄Na₂O₈S₂) has a molecular weight of 314.20. It is commonly isolated and sold as a monohydrate (C₆H₄Na₂O₈S₂·H₂O), with a Tiron molecular weight of 332.20. The value 294.22 refers to the acid form or an alternative calculation basis.

Q: How should I prepare and store Tiron solutions?

A: Due to its high solubility, Tiron can be easily dissolved in water or buffer. For long-term stability, it is recommended to prepare smaller aliquots of stock solutions and store them at -20°C, preferably under an inert atmosphere like nitrogen to prevent oxidation. Avoid repeated freeze-thaw cycles.

Q: Is Tiron cell-permeable?

A: Yes, one of Tiron's advantages in biological research is its ability to cross cell membranes, allowing it to act on intracellular targets, unlike some other bulky metal chelators like EDTA.

Q: What safety precautions should I take?

A: Tiron is generally considered low toxicity (intraperitoneal LD₅₀ in mice is 6060 mg/kg). However, as with all fine chemicals, it should be handled with care. It is classified as an irritant (Xi). Always wear appropriate personal protective equipment (PPE) including gloves and safety glasses. Refer to the Safety Data Sheet (SDS) for comprehensive handling and disposal information.