Xi'an Huilin Bio-Tech Co., Ltd. is one of the leading manufacturers and suppliers of raffinose powder in China. With abundant experience, we are committed to providing high quality natural products with competitive price. Please feel free to wholesale bulk pure raffinose powder for sale here from our factory.
Xi'an Huilin Bio-tech Co., Ltd. supplies raffinose in bulk, in the pentahydrate form, with the assay basis, the water-of-crystallisation figure and the batch documentation agreed before the order is placed.
Raffinose Powder - D-(+)-Raffinose Pentahydrate
Raffinose Powder is D-(+)-raffinose pentahydrate at 98% or more by HPLC, CAS 17629-30-0 - a non-reducing trisaccharide built from galactose, glucose and fructose, supplied as a white crystalline powder for lyophilisation, biopreservation and culture media. It is recovered from beet molasses, which is the main commercial source, and purified by crystallisation.
The pentahydrate is part of the specification, not a detail. Raffinose crystallises with five molecules of water, and that water is 15.1% of the powder's mass. A formulation written on the anhydrous figure and dosed with the pentahydrate will be short by that amount. The assay on this page is stated as the pentahydrate, the anhydrous equivalent is on the same certificate, and the two numbers are not interchangeable.
Specification
| Item | Specification | Test Method |
| Assay (as D-(+)-raffinose pentahydrate) | ≥98.0% | HPLC |
| Water of crystallisation | 13.0–16.0% (theory 15.1%) | Karl Fischer / loss on drying |
| Anhydrous equivalent | Reported on the Certificate of Analysis | Calculated from the assay |
| Appearance | White crystalline powder | Visual |
| Melting range (pentahydrate) | About 80 °C | Capillary method |
| Specific optical rotation [α]D | About +105° (aqueous solution) | Polarimetry |
| pH (5% solution) | 4.5–7.0 | pH meter |
| Solubility | About 14% in water at 20 °C, rising with temperature; slightly soluble in ethanol | As per monograph |
| Clarity and colour of solution | Clear and colourless | Visual |
| Ash | ≤0.1% | Gravimetric – 550 °C |
| Lead | ≤1 mg/kg | ICP-MS |
| Arsenic | ≤1 mg/kg | ICP-MS |
| Total plate count | ≤1,000 CFU/g | Pour plate |
| Yeasts and moulds | ≤100 CFU/g | Pour plate |
| Escherichia coli | Absent per g | / |
| Salmonella | Absent per 25 g | / |
The data above is for reference only. Please request the latest Certificate of Analysis (COA) for batch-specific data.
Identity and Grade
Two CAS numbers, one product. 17629-30-0 is the pentahydrate, 512-69-6 is the anhydrous form. Both describe the same molecule at different water content. Quotations that quote one number and certificates that report the other are not in conflict - but the assay basis has to match the material shipped, or the dosing calculation will not.
Why beet molasses and not cottonseed. Raffinose can be recovered from either, but beet molasses carries it at a workable concentration and crystallises cleanly, which is why it is the commercial route. Cottonseed is the source of the raffino-oligosaccharide mixture, where raffinose and stachyose are sold together rather than separated - a different product with a different specification.
Regulatory position in China. Raffinose is approved in China as a nutrition fortifier under a 2008 announcement, but that approval attaches to a food-grade article. The grade on this page is the reagent grade, supplied for laboratory and analytical use. It is not offered as a food ingredient, a dietary supplement or a cosmetic ingredient, and it should not be substituted for the food-grade article in a formulation or on a label. The raffino-oligosaccharide mixture takes a third route - approved as a new food raw material - so the three designations are not interchangeable.
Outside China. Whether this grade can be imported and held depends on the destination and on the use it is put to. Tell us the market and the end use and we will confirm which documents we can issue - specification, COA, manufacturing route and SDS.
Stability and Handling
• Acid. Little decomposition at pH 3.5 and 90 °C over 30 minutes, which is unusual for an oligosaccharide and is the reason it survives acidic process streams.
• Heat. Thermally stable to about 140 °C; decomposition begins around 180 °C, where it breaks into melibiose and fructose.
• Moisture. The crystalline pentahydrate stays free-flowing at 90% relative humidity, where many sugar powders cake. In the amorphous state the same material is strongly hygroscopic, so the non-caking behaviour belongs to the crystal.
• Non-reducing. No free anomeric hydroxyl, so it does not initiate Maillard browning on its own in an amino-acid-containing system.
Why Choose Huilin for Raffinose
1. The assay basis is stated on the page and on the COA. Pentahydrate by HPLC, with the anhydrous equivalent reported alongside, so a dosing calculation does not have to be guessed.
2. The water figure is a specification line. Water of crystallisation is released per batch against a range, because it is the number that moves when a customer switches between the two CAS forms.
3. Small volumes. A 25 kg minimum lets a laboratory or a pilot line work without committing to a tonne.
4. Stock, not a production schedule. Material is supplied from stock, which keeps a trial order to days rather than weeks.
5. Export and supply experience. More than 15 years of export experience, with shipments arranged on FOB, CIF, EXW or DDP terms from Shanghai, Ningbo or Guangzhou.
Raffinose or Raffino-oligosaccharides?
These are two products, not two grades of one.
This page is the single compound - one trisaccharide, one CAS number, 98% purity, recovered from beet molasses and regulated in China as a nutrition fortifier. It is specified, dosed and labelled as one substance.
The raffino-oligosaccharide mixture is raffinose and stachyose sold together, standardised on their combined content as ROS-45 or ROS-70, recovered from cottonseed and regulated as a new food raw material. If your specification is written on a combined total, that is the page you want - Cottonseed Oligosaccharide Powder.
Benefits of Raffinose in Production and Formulation
• A defined single substance. One CAS number, one assay, one anhydrous equivalent - nothing to interpret, which matters when a formulation is transferred between sites.
• Non-reducing, so it does not start browning. In an amino-acid-containing system the sugar does not initiate Maillard colour on its own.
• Stable through acid and heat. Holds through pH 3.5 at 90 °C for 30 minutes and to about 140 °C dry, so the sugar is rarely the limiting component in a process.
• A crystal that stays free-flowing. The pentahydrate does not cake at 90% relative humidity, which keeps weighing and dosing consistent to the end of the bag.
• Solubility that rises with temperature. About 14% at 20 °C, above sucrose at 80 °C, so a solution can be concentrated hot without crystallising out on cooling.
Raffinose Applications
• Lyophilisation and biopreservation: as a saccharide component of preservation and cryoprotectant formulations for biological material.
• Microbiological culture media: as a defined carbohydrate in media where a single, assayable sugar is wanted.
• Analytical work: as a reference standard where a single trisaccharide is required, including use as the calibrant for methods that distinguish raffinose from stachyose.
Final application performance depends on the assay basis used in the formulation, the water of crystallisation, the addition level, the pH and temperature of the process, the ionic environment and the storage conditions, as well as on the specification of the material supplied.
Raffinose FAQs
Q: Why does the assay specify the pentahydrate?
A: Because that is the form the material is sold and shipped in, and water is 15.1% of its mass. Reporting 98% on the pentahydrate is a statement about the powder in the bag. The anhydrous equivalent is on the same certificate for anyone building a formulation on the dry basis.
Q: How much water is in it, and why does it matter?
A: 15.1% by mass at full stoichiometry. It matters because a dosing calculation written against the anhydrous figure and weighed out as the pentahydrate lands about 15% short on a molar basis. The specification gives a range around the theoretical value so the figure can be checked batch by batch.
Q: Is raffinose the same as the cottonseed oligosaccharide?
A: No. Raffinose is a single trisaccharide with CAS 17629-30-0, regulated in China as a nutrition fortifier. The cottonseed oligosaccharide is a raffinose-plus-stachyose mixture standardised on its combined content, regulated as a new food raw material. They are sold on different assay bases and cannot substitute for one another in a registration.
Q: Is it a reducing sugar?
A: No. Raffinose carries no free anomeric hydroxyl, so it does not initiate Maillard browning on its own. At elevated temperature in acid it can hydrolyse and release reducing sugars, after which browning proceeds normally - so the useful question is where in your process that starts.
Q: Will it cake in storage?
A: The crystalline pentahydrate will not; it stays free-flowing at 90% relative humidity. Once the bag is opened it takes up moisture, so reseal it. In an amorphous state the same material is strongly hygroscopic.
Q: What is the difference between raffinose and stachyose?
A: Raffinose is a trisaccharide - galactose, glucose, fructose. Stachyose is a tetrasaccharide with one more galactose unit. Both carry α-1,6 linkages, and they are the two components of the cottonseed oligosaccharide mixture, but as single compounds they are different products with different CAS numbers.
Q: What documents come with each batch?
A: A Certificate of Analysis reporting the assay, the water of crystallisation, the anhydrous equivalent, ash, pH, optical rotation, heavy metals and microbiological results, with methods. Documentation to support import requirements in your market is provided on request, confirmed against the destination and the product category before shipment.
Raffinose Supplier
Xi'an Huilin Bio-tech Co., Ltd. supplies Raffinose Powder (CAS 17629-30-0) in bulk from 25 kg, as the pentahydrate at 98% or more by HPLC, with the assay basis, the water-of-crystallisation range and the batch documentation agreed before the order is placed. Send us your application, the assay basis your formulation is written on, your destination market and your volume; we will confirm availability, the specification sheet, the COA and the shipment arrangement. Shipments are arranged on FOB, CIF, EXW or DDP terms from Shanghai, Ningbo or Guangzhou.
For inquiries, quotations, or sample requests, please contact: sales@huilinbio-tech.com
For laboratory and analytical use only. Not for human or veterinary use. Not a food, food ingredient, dietary supplement or cosmetic ingredient, and not a pharmaceutical active substance or excipient. The information on this page is provided for evaluation by qualified technical personnel; import and possession requirements must be confirmed against the regulation that applies in your destination market.
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