Introduction to Hyocholic Acid
Hyocholic Acid (HCA), also known as γ-Muricholic Acid, is a naturally occurring bile acid predominantly found in pigs and other mammals. It has attracted significant research interest for its role in metabolic health, particularly in regulating blood sugar levels by promoting GLP-1 secretion-a hormone that supports insulin production and glucose balance-as demonstrated in diabetic mouse models. HCA also exhibits low cytotoxicity, supporting its safety for research and potential applications.
Beyond research, hyocholic acid is a key component in bile acid-based feed additives. In China, the Ministry of Agriculture approved bile acids as new feed additives in 2014 under Announcement No. 2131, permitting their production, distribution, and use within the country. They are currently approved for use in weaned piglets, freshwater fish, laying hens, and other species, with specifications governed by the national standard GB 7300.1301-2025. Our Hyocholic Acid Powder is produced from natural porcine bile through saponification, decolorization, acidification, purification, and drying, with strict quality control in a cGMP-certified facility to ensure high purity (≥98% by HPLC) and batch-to-batch consistency. Each shipment includes a complete COA, SDS, and product specification sheet.
Physical & Chemical Parameters
| Parameter | Specification |
|---|---|
| Appearance | White to off-white crystalline powder |
| Odor | Characteristic odor |
| Assay (HPLC) | ≥98.0% (typical: 98.5% – 99.5%) |
| Molecular Formula | C₂₄H₄₀O₅ |
| Molecular Weight | 408.57 g/mol |
| Melting Point | 188 – 189°C |
| Loss on Drying | ≤1.0% |
| Residue on Ignition | ≤0.1% |
| Heavy Metals (as Pb) | ≤10 ppm |
| Lead (Pb) | ≤1.0 mg/kg |
| Arsenic (As) | ≤1.0 mg/kg |
| Mercury (Hg) | ≤0.1 mg/kg |
| Cadmium (Cd) | ≤1.0 mg/kg |
| Total Plate Count | ≤1,000 CFU/g |
| Yeast & Mold | ≤100 CFU/g |
| E. coli | Negative |
| Salmonella | Negative (per 25g) |
| Solubility | Soluble in DMSO (42 mg/mL); soluble in ethanol; practically insoluble in water |
| Storage | −20°C (long-term); 4°C (short-term) |
Note: Batch-specific COA is available upon request.
Hyocholic Acid benefits
- Promotes GLP-1 Secretion & Glucose Homeostasis: Hyocholic acid activates TGR5 and inhibits FXR in enteroendocrine cells, stimulating GLP-1 secretion and improving glucose tolerance. Animal studies show that oral HCA (100 mg/kg/day) improves serum fasting GLP-1 levels and glucose homeostasis in diabetic mouse models.
- Supports Metabolic Health: HCA has been linked to improved glucose metabolism and insulin sensitivity, making it a promising candidate for diabetes and metabolic syndrome research.
- Low Cytotoxicity: Hyocholic acid exhibits low toxicity against human hepatoma HepG2 cells, supporting its safety for research and potential therapeutic applications.
- Natural Bile Acid Source: Derived from porcine bile, HCA is a naturally occurring compound with established biological functions in lipid metabolism and energy homeostasis.
- Research-Validated Mechanisms: HCA upregulates glucagon gene transcription and GLP-1 protein secretion in STC-1 and NCI-H716 cells at concentrations of 25–50 μM.
- Feed Additive Applications: As a component of bile acid feed additives, HCA supports growth performance and digestive health in livestock, with approval for use in weaned piglets, freshwater fish, and laying hens.
- Pharmaceutical Intermediate: Serves as a key starting material for the synthesis of ursodeoxycholic acid (UDCA) and other steroidal compounds.
- Biochemical Research Tool: Widely used as an internal standard for the quantitation of human fecal bile acids and in bile acid metabolism studies.
What is Hyocholic Acid used for?
- Dietary Supplements: Metabolic health supplements, glucose management formulas, and bile acid-based nutraceuticals
- Diabetes Research: GLP-1 secretion studies, glucose homeostasis research, and type 2 diabetes drug development
- Metabolic Health Research: Obesity and metabolic syndrome studies, lipid metabolism research, and energy homeostasis investigations
- Pharmaceutical Intermediates: Synthesis of ursodeoxycholic acid (UDCA) and other steroidal pharmaceutical compounds
- Feed Additives: Bile acid-based feed additives for weaned piglets, freshwater fish, and laying hens; comply with GB 7300.1301-2025
- Biochemical Research: Internal standard for fecal bile acid quantitation and bile acid metabolism studies
- Analytical Reference Standards: HPLC and mass spectrometry reference standard for bile acid analysis
Frequently Asked Questions (FAQ)
Q: What are the key mechanisms of action for Hyocholic Acid?
A: Hyocholic acid acts through two primary mechanisms: (1) activation of the TGR5 receptor and (2) inhibition of FXR (farnesoid X receptor) in enteroendocrine cells. This dual action promotes GLP-1 (glucagon-like peptide-1) secretion, which enhances insulin secretion and improves glucose tolerance.
Q: What is your MOQ? Can I order samples?
A: Our standard MOQ is 1 kg per product. Yes, free samples (5–20 g) are available upon request for qualified customers; shipping charges may apply for sample delivery.
Q: Can Hyocholic Acid be used in dietary supplements?
A: Hyocholic acid is primarily used in research and feed additive applications. For dietary supplement use, regulatory status varies by country. Buyers are responsible for ensuring compliance with local regulations in their target markets.
Q: Is Hyocholic Acid toxic?
A: Hyocholic acid has been shown to exhibit low cytotoxicity against human hepatoma HepG2 cells. However, as with all bioactive compounds, appropriate safety precautions should be observed during handling and formulation.
Q: Do you offer custom packaging or OEM formulation services?
A: Yes. We offer OEM/ODM services including custom packaging, custom particle sizing, and formulation development. Please contact our sales team for a detailed discussion.
Our Advantages
Your Professional Manufacturing & Sourcing Partner
We are more than a supplier - we are a comprehensive supply chain partner dedicated to high-quality ingredients, custom formulations, and finished products across multiple industries.
With years of experience in plant extracts, food additives, cosmetic raw materials, and nutritional ingredients, our team brings deep technical knowledge and market insight to every partnership.
01. Diverse Product Range
We offer a broad range of products, including food additives, plant extracts, fruit & vegetable powders, cosmetic actives, and functional food ingredients.
- Raw Materials: High-purity ingredients for food, beverage, supplement, and cosmetic applications.
- Finished Products: In-house manufacturing of capsules, softgels, gummies, liquid drops, and blended powders.
- Label Design & Packaging: Custom branding tailored to your target market.
- Formulation & R&D: Expert support to develop custom blends, proprietary formulas, and innovative products.
- Global Logistics: Reliable shipping, documentation, and customs clearance support.
04. Quality Assurance
Strict adherence to cGMP standards and full regulatory transparency. We stay current with global requirements, including FDA GRAS, EFSA, and other regional regulations, to ensure your products meet the highest safety and compliance standards.
How to contact
Contact our sales team to discuss your Hyocholic Acid Powder requirements.
Please get in touch with us at ella.zhang@huilinbio-tech.com.
References
PMID: 37336340 DOI: 10.1016/j.steroids.2023.109260
PMID: 33338411 DOI: 10.1016/j.cmet.2020.11.017
Disclaimer
This product is for manufacturing, formulation, and research use only-not for direct human consumption. Health-related statements are for informational purposes and do not constitute medical advice. Regulatory status varies by country; buyers are responsible for local compliance.
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