Xi'an Huilin Bio-Tech Co., Ltd. is one of the leading manufacturers and suppliers of dried beef bile powder in China. With abundant experience, we are committed to providing high quality natural products with competitive price. Please feel free to wholesale bulk pure dried beef bile powder for sale here from our factory.
Introduction
Dried Beef Bile Powder is a natural, animal-derived product obtained from the bile of cattle (Bos taurus). Bile is a complex digestive fluid produced by the liver, stored in the gallbladder, and released into the small intestine to emulsify and break down dietary fats. The dried powder form retains the full spectrum of bile components naturally found in fresh bovine bile.
Bovine bile is a complex biological fluid composed of approximately 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins, and 0.3% bilirubin. The core active components are bile acids, which include:
- Cholic Acid (CA) – The primary bile acid in cattle, constituting 62.9% to 83.5% of the total biliary bile acid pool
- Chenodeoxycholic Acid (CDCA) – Another primary bile acid present in significant quantities
- Deoxycholic Acid (DCA) – A secondary bile acid formed by microbial conversion
In adult cattle, the biliary bile acids are conjugated with taurine or glycine and consist mostly (>90%) of three bile acids: cholic acid (CA), chenodeoxycholic acid (CDCA), and deoxycholic acid (DCA). Cholic acid and deoxycholic acid together account for approximately 50% of the bile salts. These bile acids play an essential role in fat digestion and absorption, as well as in the absorption of fat-soluble vitamins (A, D, E, and K).
Bile products have been used in traditional medicine for centuries across various cultures. In the modern era, dried beef bile powder is widely recognized as a valuable ingredient in dietary supplements, functional foods, cosmetics, and pharmaceutical research.
Our Advantage: Our manufacturing facility is located in Xi'an, Shaanxi province - a strategic hub for China's natural ingredient and biotech industries. All products are manufactured in accordance with FDA-compliant quality management systems, HACCP, ISO 9001, and ISO 22000 standards, with full traceability from raw material sourcing through processing and final release.
Certificate of Analysis (COA)
| Parameter | Specification |
|---|---|
| Appearance | Yellowish-green to brownish powder |
| Odor | Partly sweet, partly bitter, characteristic |
| Cholic Acid Content (Titration) | ≥45% of dry matter (typical 45–55%) |
| Loss on Drying | ≤5.0% |
| pH (5% aqueous solution) | 5.5 – 7.5 |
| Heavy Metals (Total) | ≤20 ppm |
| Lead (Pb) | ≤2 ppm |
| Arsenic (As) | ≤1 ppm |
| Total Plate Count | ≤1,000 CFU/g |
| Yeast & Mold | ≤100 CFU/g |
| Coliform Organisms | Negative |
| E. coli | Negative |
| Salmonella | Negative |
| Staphylococcus aureus | Negative |
| Solubility (2% in BGB Broth) | Total |
| Storage | 2–8°C, protected from light and moisture |
Note: Batch-specific COA is available upon request.
Process Flow
Our Dried Beef Bile Powder is produced from fresh bovine bile sourced from healthy cattle in controlled farming regions, subject to strict veterinary oversight and BSE/TSE‑free certification to ensure animal health and traceability.
- Raw material collection – Fresh bovine bile is collected from approved abattoirs under sanitary conditions, with full traceability to the source herd.
- Defatting and filtration – The bile is subjected to a defatting process to remove excess lipids, followed by filtration to eliminate insoluble impurities.
- Dehydration / Drying – The purified bile is processed using a low‑temperature dehydration method that preserves the bile's natural constituents, including conjugated bile acids, bile pigments, cholesterol, and mucin.
- Milling and sieving – The dried product is milled to a fine powder and sieved to ensure uniform particle size.
- Quality control testing – Each batch is sampled and tested for cholic acid content, loss on drying, heavy metals, and microbiological limits.
- Packaging and storage – Finished powder is packaged in moisture‑barrier, light‑protective containers and stored under refrigerated conditions (2–8°C).
Chemical Composition & Active Components
Bovine bile is a complex biological fluid. It is composed of approximately 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins, and 0.3% bilirubin. The core active components are bile acids, which include:
- Cholic Acid (CA) – The primary bile acid in cattle, constituting approximately 62.9% to 83.5% of the total biliary bile acid pool
- Chenodeoxycholic Acid (CDCA) – Another primary bile acid present in significant quantities
- Deoxycholic Acid (DCA) – A secondary bile acid formed by microbial conversion of cholic acid in the intestine
In bovine bile, cholic acid and deoxycholic acid together account for approximately 50% of the bile salts. The bile acids in cattle are predominantly conjugated with taurine or glycine, with the three major bile acids (CA, CDCA, and DCA) comprising over 90% of the total biliary bile acid composition.
Expanded Mechanism of Action
Beyond the fundamental role of fat emulsification, bile acids exert their physiological effects through three distinct mechanisms:
- Physical Emulsification & Solubilization – In the intestinal tract, bile acids function as biological surfactants (natural detergents), emulsifying dietary fats into smaller droplets. This increases the surface area available for pancreatic lipases to break down fats into fatty acids and glycerol. Bile acids also form absorbable micelles that solubilize fat digestion products, which is essential for the absorption of triglycerides and fat‑soluble vitamins.
- Enterohepatic Circulation – Following their role in digestion, approximately 95% of bile acids are reabsorbed in the distal ileum and return to the liver via the portal vein, enabling efficient recycling and maintaining the bile acid pool.
- Signaling Molecule Function – Bile acids are not merely digestive agents; they are also important signaling molecules. They activate nuclear receptors such as the Farnesoid X Receptor (FXR), which serves as a master regulator integrating bile acid signaling with lipid, glucose, and inflammatory pathways to maintain metabolic homeostasis. Through FXR and the G protein‑coupled membrane receptor TGR5, bile acids regulate glucose and lipid metabolism, modulate inflammatory processes, and influence vascular function. Activation of FXR signaling can reduce lipogenesis and inhibit gluconeogenesis to alleviate metabolic diseases.
Dried Beef Bile Powder benefits
1. Enhances Absorption of Fat‑Soluble Vitamins
Bile acids are essential for the absorption of fat‑soluble vitamins A, D, E, and K. Supplementation with bile powder can help ensure optimal nutrient bioavailability, particularly for individuals with compromised bile production.
2. Supports Liver and Gallbladder Health
Dried beef bile powder promotes healthy bile flow and aids natural detoxification processes. It is particularly beneficial for individuals with reduced bile production or compromised digestive capacity, helping to relieve bloating, gas, and discomfort.
3. Supports Healthy Fat Metabolism
Bile salts support the conversion of fats into energy and contribute to the maintenance of healthy cholesterol levels. The body uses bile acids to manufacture essential vitamins and hormones.
4. Supports Gut Microbiota Balance
Bile possesses natural antimicrobial properties. Supplementation with bovine bile powder may help maintain a healthy balance of bacteria in the small intestine. For individuals with Small Intestinal Bacterial Overgrowth (SIBO), bovine bile may help regulate upper gastrointestinal flora.
5. Supports Post‑Cholecystectomy Digestion
For individuals who have undergone gallbladder removal, the liver continues to produce bile but lacks a storage and concentration mechanism. This can result in insufficient digestive capacity after high‑fat meals. Exogenous bile powder supplementation directly provides bile acids, effectively alleviating symptoms such as indigestion, nausea, bloating, and right‑side heaviness.
6. Anti‑Inflammatory Properties
Cattle bile has been shown to possess anti‑inflammatory, antipyretic (fever‑reducing), and cholagogic (bile‑flow promoting) effects. Research has demonstrated that fermented cattle bile has an anti‑inflammatory effect by inhibiting the NLRP3 inflammasome pathway.
7. Traditional Wellness Applications
In traditional medicine systems, bovine bile has been used for centuries. It is considered bitter and cold in nature, with properties including clearing heat, detoxifying, promoting bile flow, reducing jaundice, relieving swelling and pain, and improving vision. It has been used to treat conditions such as red and swollen eyes, hemorrhoidal swelling and pain, jaundice, and constipation.
8. GRAS Status and Global Acceptance
Dried beef bile powder is affirmed as GRAS by the U.S. FDA for use as a direct food ingredient. It meets USP specifications and is widely accepted in dietary supplements, functional foods, and cosmetics worldwide.
What is Dried Beef Bile Powder used for?
1. Dietary Supplements – Used in digestive health formulas, fat metabolism support, gallbladder health supplements, and bile salt replacement products for individuals without proper gallbladder function.
2. Functional Foods – Incorporated into foods as a surfactant and emulsifier; permitted in cheese at levels not exceeding 0.002% under FDA regulations.
3. Cosmetics and Personal Care – Used in anti‑aging skincare formulations, inflammation‑reducing creams, and skin rejuvenation products for its conditioning properties.
4. Animal Nutrition – Added to livestock and companion animal feeds to support fat digestion, liver health, and overall metabolic function.
5. Pharmaceutical Research – Used as a research reagent and as a source material for the extraction of purified bile acids (cholic acid, deoxycholic acid, chenodeoxycholic acid).
6. Traditional Medicine – Used in various traditional medicine systems for its heat‑clearing, detoxifying, and digestive support properties.
7. Microbiology & Laboratory Research – In bacteriological culture media, ox bile (oxgall) is used as a selective agent for the isolation and identification of intestinal pathogens, including Escherichia coli, Yersinia enterocolitica, Aeromonas hydrophila, and Vibrio species.
8. Selective Culture Media Components – Used in De Man, Rogosa, and Sharpe (MRS) broth and other media for microbial growth testing under high bile salt conditions and for studying the effects of bile salts on specific bacterial strains.
Frequently Asked Questions (FAQ)
Q: What is the difference between dried beef bile powder and purified bile acid extracts?
A: Dried beef bile powder is the whole, unfractionated dried product of bovine bile, containing the full spectrum of bile components - conjugated bile acids (cholic acid, deoxycholic acid, taurocholic acid, glycocholic acid), bile pigments, cholesterol, mucin, and other natural constituents. Purified bile acid extracts, by contrast, are refined products containing specific isolated bile acids (e.g., cholic acid, deoxycholic acid, ursodeoxycholic acid) with higher purity but without the full complement of natural bile components. Dried beef bile powder is generally used in dietary supplements, functional foods, and cosmetics where the full spectrum of bile constituents is desired.
Q: What are the critical quality control parameters for dried beef bile powder?
A: Key parameters include: cholic acid content (typically ≥42%–46% by titration), loss on drying (≤5.0%), heavy metals (total ≤20 ppm), microbiological limits (TPC ≤1,000 CFU/g, pathogens negative), appearance (brown to yellowish-green powder), and solubility. A complete COA is provided with every shipment.
Q: How should dried beef bile powder be stored, and what is its shelf life?
A: Dried beef bile powder is hygroscopic (absorbs moisture from the air) and should be stored in a tightly sealed container, protected from light and moisture, at 2–8°C (refrigerated). Under these conditions, shelf life is 24–36 months. Avoid repeated opening and closing of bulk containers in high‑humidity environments.
Q: Is dried beef bile powder compliant with global regulatory standards?
A: Yes. Dried beef bile powder (ox bile extract) is affirmed as Generally Recognized as Safe (GRAS) by the U.S. FDA under 21 CFR §184.1560 for use as a direct food ingredient. It meets the specifications of the U.S. Pharmacopeia (USP) and is also recognized in the European Union and other major markets for use in dietary supplements and food applications.
Q: What is the maximum permitted use level in food applications?
A: Under FDA regulations, ox bile extract is permitted in food at levels not exceeding good manufacturing practice. For cheese (as defined in §170.3(n)(5)), the maximum level is 0.002% as served. For other food applications, use levels should follow current good manufacturing practice.
Q: What packaging options are available for dried beef bile powder?
A: Aluminum foil bags with desiccant (1–5 kg), sealed HDPE drums with moisture barrier (10–25 kg), fiber drums (25–50 kg), and customized packaging (bulk bags, private‑label retail packaging). All packaging is moisture‑resistant, light‑protective, and suitable for refrigerated shipping.
Q: Is dried beef bile powder suitable for vegan or vegetarian products?
A: No. Dried beef bile powder is derived from cattle and is therefore not suitable for vegan or vegetarian products. It is an animal‑derived ingredient and should be labeled accordingly.
Why Choose Us
Your Professional Manufacturing & Sourcing Partner
We are more than a supplier - we are a comprehensive supply chain partner dedicated to high-quality ingredients, custom formulations, and finished products across multiple industries.
- FDA-Compliant Manufacturing in Xi'an – Our dried beef bile powder is produced in state‑of‑the‑art facilities adhering to strict quality management systems, including HACCP and ISO 9001/ISO 22000 certification. Full traceability from raw material through processing, drying, and final release.
- Dried beef bile powder (ox bile extract, CAS 8008-63-7) is affirmed as Generally Recognized as Safe (GRAS) by the U.S. Food and Drug Administration under 21 CFR §184.1560 for use as a direct food ingredient. Food‑grade ox bile extract must meet the specifications of the U.S. Pharmacopeia (USP). The ingredient is used as a surfactant in food in accordance with good manufacturing practice, with a maximum level of 0.002% in cheese.
- Rigorous Quality Control – Each batch undergoes comprehensive testing: cholic acid content (titration), loss on drying (≤5.0%), heavy metals (ICP‑MS), microbiological panel (TPC, yeast/molds, pathogens), and appearance. COA provided with every shipment.
- Competitive Pricing & Flexible MOQs – Direct raw material sourcing and efficient production capacity enable competitive pricing. MOQ from 25 kg to full container loads (FCL). Sample orders (1–5 kg) available for laboratory evaluation.
- Comprehensive Regulatory Support – Full documentation for product registration: COA, TDS, SDS, MSDS, non‑GMO certificate, BSE/TSE‑free certificate, allergen declaration, FDA GRAS compliance reference (21 CFR §184.1560), and ISO certificates.
- Reliable Global Logistics – Established distribution networks across North America, Europe, Asia‑Pacific, and the Middle East with refrigerated shipping (2–8°C) where required. All packaging is moisture‑resistant and light‑protective.
- Dedicated Technical Support – In‑house team provides formulation guidance, custom grade development, stability testing support, and regulatory assistance for global product registration.
How to contact
Ready to incorporate high‑quality Dried Beef Bile Powder into your supplement, functional food, or cosmetic product line?
Contact us today for:
- Certificate of Analysis (COA) and Material Safety Data Sheet (MSDS)
- Custom packaging options (moisture‑protective, private‑label)
- Free lab samples for formulation testing and batch qualification
📧 Please contact us at: ella.zhang@huilinbio-tech.com
For custom grade requests or technical support regarding cholic acid content specifications, regulatory compliance, or formulation guidance, please mention your application in the subject line for immediate handling by our technical team. We respond to all inquiries within 12–24 business hours.
References
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