Introduction
Broccoli Extract Powder is a high-quality, standardized botanical ingredient derived from the seeds or young sprouts of Brassica oleracea L. var. italica. It is naturally rich in glucoraphanin, a stable glucosinolate precursor that is enzymatically converted to sulforaphane-a bioactive isothiocyanate with well-documented antioxidant and cytoprotective properties. Sulforaphane is formed through the hydrolytic action of myrosinase, an enzyme present in both the plant tissue and the gut microbiome.
This extract has gained significant attention in the nutraceutical and functional food industries due to its ability to support cellular defense mechanisms, promote phase II detoxification enzyme activity, and contribute to overall wellness. Our Broccoli Extract Powder is manufactured under strict quality control standards, ensuring consistent potency, purity, and bioavailability across every batch.
Certificate of Analysis (COA)
| Item | Specification | Result | Method |
|---|---|---|---|
| Physical Description | |||
| Appearance | Light yellow to yellow-brown fine powder | Conforms | Visual |
| Odor & Taste | Characteristic | Conforms | Organoleptic |
| Particle Size | 100% pass 80 mesh | Conforms | Screening |
| Chemical Tests | |||
| Glucoraphanin | ≥10.0% (HPLC) | 10.80% | HPLC |
| Sulforaphane | ≥1.0% (HPLC) | 1.05% | HPLC |
| Identification | Positive | Positive | TLC |
| Loss on Drying | ≤5.0% | 3.42% | USP <731> |
| Ash Content | ≤5.0% | 3.10% | USP <281> |
| Heavy Metals | |||
| Total Heavy Metals | ≤10 ppm | Conforms | ICP-MS |
| Lead (Pb) | ≤2.0 ppm | Conforms | ICP-MS |
| Arsenic (As) | ≤1.0 ppm | Conforms | ICP-MS |
| Cadmium (Cd) | ≤1.0 ppm | Conforms | ICP-MS |
| Mercury (Hg) | ≤0.1 ppm | Conforms | ICP-MS |
| Microbiological Control | |||
| Total Plate Count | ≤10,000 cfu/g | Conforms | USP <2021> |
| Yeast & Mold | ≤1,000 cfu/g | Conforms | USP <2021> |
| E. coli | Negative | Negative | USP <2022> |
| Salmonella | Negative | Negative | USP <2022> |
| Conclusion | Conforms to specification |
Note: Batch-specific COA is available upon request.
Process Flow of Broccoli Sprout Extract Powder
- Raw Material Sourcing – High-quality broccoli seeds/sprouts are sourced from certified organic or conventional farms with traceability.
- Cleaning & Drying – Raw materials are thoroughly cleaned to remove dirt and debris, then dried to reduce moisture content.
- Grinding/Milling – Dried seeds or sprouts are ground into a fine powder using specialized milling equipment.
- Extraction – The powdered material undergoes solvent extraction (water or ethanol) to isolate bioactive compounds such as glucoraphanin and sulforaphane precursors.
- Filtration & Concentration – The extract is filtered to remove insoluble residues and concentrated under controlled conditions.
- Drying – Concentrated extract is dried using low-temperature vacuum drying or spray drying to preserve thermolabile bioactives.
- Milling & Sieving – Dried extract is milled and sieved to achieve uniform particle size (100% pass 80 mesh).
- Quality Control Testing – Each batch is tested for active ingredient content (HPLC), heavy metals, microbiological purity, and physical parameters.
- Packaging – The final powder is packaged in food-grade containers (foil bags or fiber drums) under controlled conditions to maintain stability.
Mechanism of Action
Broccoli Extract Powder exerts its biological effects primarily through its active metabolite, sulforaphane. Upon ingestion, glucoraphanin-the stable precursor present in the extract-is hydrolyzed by myrosinase (from plant tissue or gut microbiota) to release sulforaphane. Sulforaphane then activates the transcription factor NF-E2-related factor 2 (Nrf2), a master regulator of cellular antioxidant and detoxification responses. Nrf2 binds to antioxidant-response elements (AREs) in the promoter regions of target genes, promoting the transcription of phase II detoxification enzymes such as glutathione-S-transferase and NAD(P)H dehydrogenase [quinone] 1 (NQO1). This upregulation enhances the cell's capacity to neutralize reactive oxygen species, detoxify carcinogens, and maintain redox homeostasis. Additionally, sulforaphane has been shown to down-regulate pro-inflammatory mediators such as COX-2, IL-6, and TNF-α, contributing to its anti-inflammatory properties.
Broccoli Extract Powder benefits
- Powerful Antioxidant Support: Broccoli sprout extract powder (BSEP) demonstrated four times higher oxygen radical absorbance capacity (ORAC) than pure sulforaphane standard, indicating exceptional antioxidant capacity.
- Cellular Detoxification: Activates Nrf2-mediated phase II detoxification enzymes, promoting the elimination of harmful xenobiotics and reactive carcinogens.
- Liver Health Support: A randomized controlled trial demonstrated that daily intake of glucoraphanin-enriched broccoli sprout supplements significantly improved serum ALT levels in healthy middle-aged adults with high-normal hepatic biomarkers.
- Neuroprotective Properties: BSEP exhibited neuroprotective effects in amyloid-beta-induced Alzheimer's disease models, enhancing memory and learning retention while inhibiting acetylcholinesterase activity.
- Anti-Inflammatory Activity: Down-regulates pro-inflammatory gene expression (COX-2, IL-6, TNF-α) in activated macrophages.
- Immune System Support: A clinical study showed that a broccoli seed extract containing sulforaphane precursor reduced the number of cold symptom days in susceptible adults.
- Metabolic Health: Clinical trials have observed improvements in fasting glucose, glycated hemoglobin, and triglyceride profiles with broccoli sprout extract supplementation.
What is Broccoli Extract Powder used for?
- Dietary Supplements: Capsules, tablets, softgels, and powder blends for daily antioxidant and detoxification support.
- Functional Foods & Beverages: Fortified smoothies, protein shakes, energy bars, and wellness drinks.
- Cosmetic & Personal Care: Skincare formulations (serums, creams, masks) targeting anti-aging, anti-pollution, and skin protection.
- Sports Nutrition: Post-exercise recovery formulations to support muscle repair and reduce oxidative stress.
- Pharmaceutical Intermediates: As a raw material for research and development of sulforaphane-based therapeutic candidates.
Frequently Asked Questions (FAQ)
Q: What is the difference between glucoraphanin and sulforaphane?
A: Glucoraphanin is the stable precursor glucosinolate found in broccoli, while sulforaphane is the bioactive isothiocyanate produced when glucoraphanin is hydrolyzed by the enzyme myrosinase. Our Broccoli Extract Powder is standardized to glucoraphanin content, ensuring stability during storage and processing.
Q: Is Broccoli Extract Powder water-soluble?
A: Yes, Broccoli Extract Powder is soluble in water, methanol, and other organic solvents, making it suitable for a wide range of formulation applications including beverages and aqueous-based systems.
Q: What is the recommended dosage?
A: Dosage recommendations vary depending on the intended application and target population. Typical dietary supplement dosages range from 100–600 μmol of glucoraphanin or sulforaphane equivalents per day. We recommend consulting with a formulation specialist for application-specific guidance.
Q: Is this product suitable for vegetarian/vegan formulations?
A: Yes, our Broccoli Extract Powder is plant-derived and suitable for vegetarian and vegan product formulations.
Q: What certifications are available?
A: Our Broccoli Extract Powder is manufactured in facilities that are ISO, HACCP, KOSHER, and HALAL certified, with GMP-compliant production processes.
Why Choose Us
Your Professional Manufacturing & Sourcing Partner
We are more than a supplier - we are a comprehensive supply chain partner dedicated to high-quality ingredients, custom formulations, and finished products across multiple industries.
- Professional Manufacturer: Xi'an Huilin Bio-tech Co., Ltd. is a high-tech biotechnology company specializing in the production, R&D, and export of natural plant extracts.
- Quality Assurance: Every batch undergoes rigorous testing for active ingredients (HPLC), heavy metals (ICP-MS), and microbiological purity to ensure consistent quality and safety.
- Customization Capabilities: We offer flexible specifications, packaging options, and custom formulations to meet diverse customer requirements.
- Supply Chain Reliability: With established supply chains and robust production capacity, we ensure timely delivery and stable supply for bulk orders.
- Competitive Pricing: Direct factory pricing without intermediary markups.
- Technical Support: Our experienced R&D team provides formulation guidance and technical support throughout your product development process.
- Global Compliance: Products are manufactured in compliance with international quality standards and regulatory requirements.
How to contact
For inquiries, quotations, or sample requests, please contact:
Email: ella.zhang@huilinbio-tech.com
We look forward to partnering with you to bring high-quality Broccoli Extract Powder to your products.
References
- National Cancer Institute. Broccoli Sprout/Broccoli Seed Extract Supplement. NCI Thesaurus Code C128293. Upon administration of the broccoli sprout/broccoli seed extract, sulforaphane activates the transcription factor NF-E2-related factor 2 (Nrf2)... which binds to and activates antioxidant-response elements (AREs).
- Endogenous extraction yielded high quality sulforaphane from broccoli sprouts unveils potent antioxidant and anti-Alzheimer's activities. Heliyon. 2025;11(4):e42673. BSEP showed four times higher ORAC than the SFN standard. It also reduced inflammatory responses by down-regulating COX-2, IL-6, and TNF-α gene expression. BSEP exhibited neuroprotective properties in amyloid-beta-induced Alzheimer's disease mice.
- Effects of broccoli sprout supplements enriched in glucoraphanin on liver functions in healthy middle-aged adults with high-normal serum hepatic biomarkers: A randomized controlled trial. Frontiers in Nutrition. 2022;9:1077271. Glucoraphanin significantly improved serum ALT levels at 24 weeks compared to placebo supplements.
- Fahey JW, Zhang Y, Talalay P. Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc Natl Acad Sci USA. 1997;94(19):10367-10372. Extracts of 3-day-old broccoli sprouts were highly effective in reducing the incidence, multiplicity, and rate of development of mammary tumors in rats.
- Houghton CA, Fassett RG, Coombes JS. Sulforaphane: translational research from laboratory bench to clinic. Nutr Rev. 2013;71(11):709-726. Sulforaphane is a significant inducer of nuclear factor erythroid 2-related factor 2 (Nrf2), the transcription factor that activates the antioxidant response.
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