HEPES Buffer Powder

Our Pharmaceutical Grade HEPES Powder is manufactured under strict cGMP conditions to meet the most demanding requirements of modern bioprocessing and cell culture applications. With ultra-low endotoxin levels (≤0.005 EU/mg) and excellent batch-to-batch consistency, it provides reliable buffering capacity for sensitive biological systems where pH stability is critical.

This high-purity HEPES buffer is specifically designed for cell culture, molecular biology, and diagnostic applications requiring superior performance and regulatory compliance.

1. Physical & Chemical Properties:

• Purity: ≥99.5% (HPLC)
• Endotoxin: ≤0.005 EU/mg (Cell Culture Grade)
• Absorbance (1M solution): A260 ≤ 0.05, A280 ≤ 0.05
• Heavy Metals: ≤0.0001%
• Solubility: ≥50 mg/mL in water (20°C)

2. Biological Testing:

• DNase/RNase Activity: Not detected
• Microbial Limits: ≤10 CFU/g
• Sterility Testing: Available upon request

3. Specialty Grades:

• Cell Culture Grade: Endotoxin ≤0.005 EU/mg, optimized for mammalian cell culture
• Molecular Biology Grade: DNase/RNase free, for sensitive molecular applications
• Diagnostic Grade: Meets IVD regulatory requirements, ISO 13485 certified

1. Cell Culture & Bioprocessing:

Mammalian Cell Culture: 10-25 mM concentration for pH maintenance in CO₂ incubators

Stem Cell Applications: Demonstrated compatibility with sensitive stem cell cultures

Viral Vector Production: Used in lentiviral and AAV production processes

2. Molecular Biology:

PCR & qPCR: Provides stable pH in enzyme reaction buffers

Protein Biochemistry: Excellent for protein purification and crystallization

Electrophoresis: Alternative to Tris buffers in certain separation techniques

3. Diagnostic Applications:

IVD Reagents: Component of buffer systems in clinical diagnostic kits

Point-of-Care Testing: Stable buffering in lateral flow assays

COVID-19 Test Kits: Used in sample processing and reaction buffers

4. Preparation Guidelines:

1M Stock Solution: Dissolve 23.83g in 80mL water, adjust to desired pH, bring to 100mL

Sterilization: 0.1μm filtration or autoclaving (solution only)

Storage: 2-8°C for long-term storage; stable at room temperature for 6 months

The product packaging adopts the products manufactured by advanced enterprises to prevent overdue and certificates packaging

If you use our products for the first time, we will provide you with a sample service

Due to the large price fluctuations in the chemical market, the price of individual products may fluctuate in different time periods. Please contact us before purchasing to obtain the latest quotation

We are a raw material supplier from China. Our main categories: are plant extracts, cosmetic raw materials, food additives, pharmaceutical intermediates, etc., with preferential factory direct sales prices. If you want to know more about our hepes powder, please contact us through our website or email. Our customer team will always be at your service and answer any questions or concerns you may have.

Our common email addresses are ella. zhang@huilinbio-tech.com Welcome to contact us; Offline, you can come to the site to see the factory. Our factory is located in Room 706, Building B, Fengze Science Park, No. 170, West Street, Yanta District, Xi'an, Shaanxi Province.

Why choose us?

• Our products meet and exceed the most stringent quality standards.
• We are doing our best to create and promote the company's brand, open up the market and build up our image.
• We are experts in the Cosmetic Raw Materials industry.
• We are grateful for the enlightenment and inspiration of our rivals and the tolerance and giving of the society.
• Our products are backed by extensive research and development.
• We rely on the quality of our products to improve the company's reputation and maintain the company's overall image.
• We are dedicated to helping our customers succeed.
• Our strong sense of social responsibility makes us more aware of the meaning of rigor and prudence.
• Trust us to provide you with the best Cosmetic Raw Materials in the market.
• We will firmly grasp the central position of innovation in the overall reform and development, and continue to enhance our Hepes Powder technology.

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